Journal: European Journal of Human Genetics

Article Title: Genomic and clinical characteristics of six patients with partially overlapping interstitial deletions at 10p12p11

doi: 10.1038/ejhg.2011.71

Figure Lengend Snippet: Schematic illustration of chromosome 10 displaying the deletions at 10p12p11 in patients 1–6 and patient by Shadadpuri et al5 detected by microarray using the Ensembl browser. Deleted regions for the respective cases are shown as black bars. Smallest region of overlap (SRO) is indicated by vertical dotted lines. Positions are according to NCBI36 (hg18).

Article Snippet: Confirmation of the deletion and parental analysis of patient 5 was carried out by using Cytochip v2.0 microarray (Bluegnome, Cambridge, UK) and data analysis with BlueFuse for Microarrays v.3.5 (Bluegnome, Cambridge, UK).

Techniques: Microarray

Journal: Journal of Translational Medicine

Article Title: LncRNA-Gm9795 promotes inflammation in non-alcoholic steatohepatitis via NF- \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\kappa {}$$\end{document} κ B/JNK pathway by endoplasmic reticulum stress

doi: 10.1186/s12967-021-02769-7

Figure Lengend Snippet: LncRNA-gm9795 is highly expressed in liver tissues of NASH mice and cell models: a LncRNA-gm9795 mainly in the nucleus in control group; b . LncRNA-gm9795 mainly in the nucleus in MCD+LPS group; c LncRNA-gm9795 mainly in the nucleus in PA group; d Three Open Read Frames of LncRNA-gm9795 predicted in the database showed no coding capacity; e LncRNA-gm9795 is highly expressed in NASH cell models built by MCD+LPS in AML12; f LncRNA-gm9795 is highly expressed in NASH cell models built by PA in AML12; g LncRNA-gm9795 is highly expressed in NASH cell models built by MCD+LPS in mouse primary hepatocytes; h LncRNA-gm9795 is highly expressed in NASH cell models built by PA in mouse primary hepatocytes; i LncRNA-gm9795 is highly expressed in NASH mouse models; (NS: Nonsense, *P < 0.05, **P < 0.01,***P < 0.001)

Article Snippet: Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) was used to study the profiles of mouse lncRNAs and mRNA in the liver.

Techniques: Control

Journal: Journal of Translational Medicine

Article Title: LncRNA-Gm9795 promotes inflammation in non-alcoholic steatohepatitis via NF- \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\kappa {}$$\end{document} κ B/JNK pathway by endoplasmic reticulum stress

doi: 10.1186/s12967-021-02769-7

Figure Lengend Snippet: lncRNA-gm9795 did not affect lipid accumulation in NASH: a Fluorescence imaging shows there was no significant change in lipid deposition after interference with lncRNA-gm9795 in MCD+LPS model; b Fluorescence imaging shows there was no significant change in lipid deposition after interference with lncRNA-gm9795 in PA model; c Fluorescence imaging shows there was no significant change in lipid deposition after overexpressing lncRNA-gm9795 in NASH model

Article Snippet: Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) was used to study the profiles of mouse lncRNAs and mRNA in the liver.

Techniques: Fluorescence, Imaging

Journal: Journal of Translational Medicine

Article Title: LncRNA-Gm9795 promotes inflammation in non-alcoholic steatohepatitis via NF- \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\kappa {}$$\end{document} κ B/JNK pathway by endoplasmic reticulum stress

doi: 10.1186/s12967-021-02769-7

Figure Lengend Snippet: Knocking down LncRNA-gm9795 significantly decreased the expression of inflammation factors in NASH: a Interference efficiency of siRNA-gm9795 in MCD+LPS model; b In MCD+LPS model, the mRNA expression of TNF \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\alpha {}$$\end{document} α was decreased after knocking down gm9795; c In MCD+LPS model, the mRNA expression of IL-6 was decreased after knocking down gm9795; d In MCD+LPS model, the mRNA expression of IL-1 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\beta {}$$\end{document} β was decreased after knocking down gm9795; e Interference efficiency of siRNA-gm9795 in PA model; f. In PA model, the mRNA expression of TNF \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\alpha {}$$\end{document} α was decreased after knocking down gm9795;g. In PA model, the mRNA expression of IL-6 was decreased after knocking down gm9795; h In PA model, the mRNA expression of IL-1 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\beta {}$$\end{document} β was decreased after knocking down gm9795; i Relative gray analysis of protein expression of inflammation factors with gm9795 interference in MCD+LPS model; j The protein expression of inflammation factors was decreased after knocking down gm9795 in MCD+LPS model; k The protein expression of inflammation factors was decreased after knocking down gm9795 in PA model; l Relative gray analysis of protein expression of inflammation factors with gm9795 interference in PA model; (*P < 0.05, **P < 0.01,***P < 0.001)

Article Snippet: Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) was used to study the profiles of mouse lncRNAs and mRNA in the liver.

Techniques: Expressing

Journal: Journal of Translational Medicine

Article Title: LncRNA-Gm9795 promotes inflammation in non-alcoholic steatohepatitis via NF- \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\kappa {}$$\end{document} κ B/JNK pathway by endoplasmic reticulum stress

doi: 10.1186/s12967-021-02769-7

Figure Lengend Snippet: LncRNA-gm9795 significantly increased the expression of inflammatory factors in NASH: a The efficiency of gm9795 overexpression plasmid; b The mRNA expression of TNF \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\alpha {}$$\end{document} α increased after the overexpression of gm9795; c The mRNA expression of IL-6 increased after the overexpression of gm9795; d The mRNA expression of IL-1 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\beta {}$$\end{document} β increased after the overexpression of gm9795; e The protein expression of inflammatory factors increased after the overexpression of gm9795; f Relative gray analysis of protein expression of inflammation factors with gm9795 overexpression; (*P < 0.05, **P < 0.01,***P < 0.001)

Article Snippet: Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) was used to study the profiles of mouse lncRNAs and mRNA in the liver.

Techniques: Expressing, Over Expression, Plasmid Preparation

Journal: Journal of Translational Medicine

Article Title: LncRNA-Gm9795 promotes inflammation in non-alcoholic steatohepatitis via NF- \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\kappa {}$$\end{document} κ B/JNK pathway by endoplasmic reticulum stress

doi: 10.1186/s12967-021-02769-7

Figure Lengend Snippet: LncRNA-gm9795 significantly increased the expression of inflammation factors in NASH through NF-k κ B/JNK pathway: a The expression of NF- \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\kappa {}$$\end{document} κ B/JNK signaling proteins was decreased after gm9795 knockout in MCD+LPS model; b The expression of NF- \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\kappa {}$$\end{document} κ B/JNK signaling proteins was decreased after gm9795 knockout in PA model; c The expression of NF- \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\kappa {}$$\end{document} κ B/JNK signaling proteins was increased after the overexpression of gm9795; d Relative gray analysis of protein expression of NF- \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\kappa {}$$\end{document} κ B/JNK with gm9795 interference in MCD+LPS model; e Relative gray analysis of protein expression of NF- \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\kappa {}$$\end{document} κ B/JNK with gm9795 interference in PA model; f Relative gray analysis of protein expression of NF- \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\kappa {}$$\end{document} κ B/JNK with gm9795 overexpression; (*P < 0.05, **P < 0.01,***P < 0.001)

Article Snippet: Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) was used to study the profiles of mouse lncRNAs and mRNA in the liver.

Techniques: Expressing, Knock-Out, Over Expression

Journal: Journal of Translational Medicine

Article Title: LncRNA-Gm9795 promotes inflammation in non-alcoholic steatohepatitis via NF- \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\kappa {}$$\end{document} κ B/JNK pathway by endoplasmic reticulum stress

doi: 10.1186/s12967-021-02769-7

Figure Lengend Snippet: LncRNA-gm9795 activates ERS: a The mRNA expression of IRE1 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\alpha {}$$\end{document} α was decreased after gm9795 knockout in MCD+LPS model; b The mRNA expression of BIP was decreased after gm9795 knockout in MCD+LPS model; c The mRNA expression of CHOP had no significant change after gm9795 knockout in MCD+LPS model; d The mRNA expression of IRE1 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\alpha {}$$\end{document} α was decreased after gm9795 knockout in PA model; e The mRNA expression of BIP was decreased after gm9795 knockout in PA model; f The mRNA expression of CHOP had no significant change after gm9795 knockout in PA model; g The mRNA expression of IRE1 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\alpha {}$$\end{document} α were increased with gm9795 overexpression; h The mRNA expression of BIP were increased with gm9795 overexpression; i The mRNA expression of CHOP had no significant change with gm9795 overexpression; j The protein expression of ERS was decreased after gm9795 knockout in MCD+LPS model; k The protein expression of ERS was decreased after gm9795 knockout in PA models; l The protein expression of ERS was were increased with gm9795 overexpression; o Relative gray analysis of protein expression of ERS with gm9795 interference in MCD+LPS model; e Relative gray analysis of protein expression of ERS with gm9795 interference in PA model; f Relative gray analysis of protein expression of ERS with gm9795 overexpression; (*P < 0.05, **P < 0.01,***P < 0.001)

Article Snippet: Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) was used to study the profiles of mouse lncRNAs and mRNA in the liver.

Techniques: Expressing, Knock-Out, Over Expression

Journal: Journal of Translational Medicine

Article Title: LncRNA-Gm9795 promotes inflammation in non-alcoholic steatohepatitis via NF- \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\kappa {}$$\end{document} κ B/JNK pathway by endoplasmic reticulum stress

doi: 10.1186/s12967-021-02769-7

Figure Lengend Snippet: LncRNA-gm9795 activated NF-k κ B/JNK pathway through ERS: a The NF-k κ B/JNK pathway was activated by introducing endoplasmic reticulum stress agonists after gm9795 was knocked out in MCD+LPS model; b The NF-k κ B/JNK pathway was activated by introducing endoplasmic reticulum stress agonists after gm9795 was knocked out in PA model; c The NF-k κ B/JNK pathway was inhibited by introducing endoplasmic reticulum stress inhibitor after gm9795 was overexpressed; d Relative gray analysis of protein expression of NF \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\kappa {}$$\end{document} κ B/JNK with gm9795 interference and TG in MCD+LPS model; e Relative gray analysis of protein expression of NF \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\kappa {}$$\end{document} κ B/JNK with gm9795 interference and TG in PA model; f Relative gray analysis of protein expression of NF \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\kappa {}$$\end{document} κ B/JNK with gm9795 overexpression and TUDCA; (*P < 0.05, **P < 0.01,***P < 0.001)

Article Snippet: Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) was used to study the profiles of mouse lncRNAs and mRNA in the liver.

Techniques: Expressing, Over Expression

Journal: Journal of Translational Medicine

Article Title: LncRNA-Gm9795 promotes inflammation in non-alcoholic steatohepatitis via NF- \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\kappa {}$$\end{document} κ B/JNK pathway by endoplasmic reticulum stress

doi: 10.1186/s12967-021-02769-7

Figure Lengend Snippet: Diagram of LncRNA-Ggm9795 promoting inflammation in NASH:LncRNA-gm9795 activated NF-k κ B/JNK pathway through ERS

Article Snippet: Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) was used to study the profiles of mouse lncRNAs and mRNA in the liver.

Techniques:

Journal: Clinical and Translational Medicine

Article Title: Androgen receptor decreases the renal cell carcinoma bone metastases via suppressing the osteolytic formation through altering a novel circEXOC7 regulatory axis

doi: 10.1002/ctm2.353

Figure Lengend Snippet: CircRNA microarray and screening process. Results from circRNA microarray and bioinformatics analysis from five paired human RCC vs. RBM tissues were summarized in (A‐D). (A) Box plot demonstrated no significant differences in the distribution of intensity across 10 clinical samples. (B) Scatter plot displayed circRNAs expression correlation between RCC and RBM group. (C) Heat map generated from circRNAs microarray data reflecting circRNAs expression values in RCC and RBM groups. (D) Volcano plot showing DERs data. The red points indicate points‐of‐interest that display both large magnitude fold‐changes (x‐axis) and high statistical significance (‐log10 of p ‐value, y‐axis). The green line shows points above the line having p < 0.05 and points below the line having p > 0.05. This plot is colored such that those points having a fold‐change less than 2 (log2 = 1) are shown in gray. (E) The qRT‐PCR was performed to show 32 selected candidate circRNAs expression levels in cells. (F) The qRT‐PCR was performed on SW839 cells to show effects of manipulating efficiency of selected candidate circRNAs, ciR4#, ciR6#, ciR19#, and ciR26#. (G‐J) Interruption assays revealed that suppressing (G) ciR‐4# (hsa_circ_0001275), (H) ciR‐6# (hsa_circRNA_405974), expression cannot reverse/block the knocked‐down AR‐increased osteolytic formation in SW839 cells (scale bars, 200 μm), increasing (I) ciR‐19# (hsa_circ_0091743) expression, and (J) increasing ciR‐26# hsa_circ_0075303 expression cannot reverse/block the AR‐suppressed osteolytic formation in SW839 cell line (scale bars, 200 μm). For (E‐J), quantitation is at the right, and data are presented as Mean ± SD, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Abbreviation: N.S., not significant

Article Snippet: In the current study, five human RCC and five RCC bone metastasis tissues were deeply sequenced using Arraystar human circRNA V2.0 microarray.

Techniques: Microarray, Expressing, Generated, Quantitative RT-PCR, Blocking Assay, Quantitation Assay

Journal: Clinical and Translational Medicine

Article Title: Androgen receptor decreases the renal cell carcinoma bone metastases via suppressing the osteolytic formation through altering a novel circEXOC7 regulatory axis

doi: 10.1002/ctm2.353

Figure Lengend Snippet: The expression of circEXOC7 is regulated by DHX9. (A) The qRT‐PCR was performed on SW839 and OS‐RC‐2 cell lines to show correlation between AR and four genes related to circRNA biogenesis. Among them, ADAR2 and DHX9 have a positive correlation with AR mRNA expression. (B and C) The qRT‐qPCR was performed to show (B) knocking down DHX9, and not (C) ADAR2 gene, led to increased circEXOC7 expression in SW839 cells. (D) WB was performed on SW839 and OS‐RC‐2 cells to show that modulated AR expression also altered DHX9 expression. (E) Predicted AR binding sites on the DHX9 promoter region using website http://jaspar.genereg.net/ . (F) ChIP assay showing AR could bind to the No. 2 potential ARE on the DHX9 5′‐promoter region. (G) The wild type and mutant pGL3‐DHX9 promoter reporter constructs. (H and I) Luciferase activity after transfection of wild type or mutant DHX9 promoter reporter constructs in OS‐RC‐2 cells (H) transfected with AR‐cDNA or pWPI and SW839 cells (I) transfected with AR‐shRNA or pLKO. For (B and C), (H and I), data are presented as Mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001. Abbreviation: N.S., not significant

Article Snippet: In the current study, five human RCC and five RCC bone metastasis tissues were deeply sequenced using Arraystar human circRNA V2.0 microarray.

Techniques: Expressing, Quantitative RT-PCR, Binding Assay, Mutagenesis, Construct, Luciferase, Activity Assay, Transfection, shRNA